Maternal hair testing for the assessment of fetal exposure to drug of abuse during early pregnancy: Comparison with testing in placental and fetal remains

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• 作者：M. Falcon^a ; ^falcon@um.es ; S. Pichini^b ; J. Joya^c ; M. Pujadas^c ; A. Sanchez^a ; O. Vall^c ; ^d ; O. Garcí ; a Algar^c ; ^d ; A. Luna^a ; R. de la Torre^c ; M.C. Rotolo^b ; M. Pellegrini^b
• 关键词：CYP450 ; cytochrome P450 ; MAM ; 6-monoacetylmorphine ; MOR ; morphine ; COC ; cocaine ; BEG ; benzoylecgonine ; MDMA ; 3 ; 4-methylenedioxymethamphetamine ; MA ; methamphetamine ; THC ; delta-9-tetrahydrocannabinol ; THCCOOH ; 11-nor-D9-tetrahydrocannabinol-9-carboxylic ac
• 刊名：Forensic Science International
• 出版年：2012
• 期刊代码：104_03790738
• 类别：med
• 出版时间：10 May, 2012
• 卷：218
• 期：1-3
• 页码：92-96
• 文件大小：187 K

摘要

Drug use by pregnant women in the first trimester of pregnancy and subsequent fetal exposure during early gestation can be assessed only by repetitive/systematic maternal blood/urine analysis or segmental hair analysis. No evidence of any relationship between maternal/fetal exposure during this specific period of gestation has been demonstrated to date in a human model.

Methods

To clarify drugs toxicokinetics and transplacental passage during early pregnancy, the presence of the most widely used recreational drugs of abuse and metabolites was investigated in the proximal 4 cm hair segments of women undergoing voluntary termination of pregnancy (n = 280) during the 12th week of gestation and the results were compared to those from placenta and fetal tissue samples in order to verify whether maternal hair testing can reflect fetal exposure and, if so, to what extent. Hair, placenta and fetal remains were analyzed by validated gas chromatography mass spectrometry assays.

Results

Eighty one positive hair samples were identified: 60 were positive for cannabis (74.1%), 28 for cocaine (34.6%), 7 for opiates (8.6%), 3 for MDMA (3.7%) and 18.5%were positive for more than one drug. The positive hair test results were confirmed in placenta/fetal tissues in 10 cases out of 60 for cannabis (16. 7%); in 7 out of 28 for cocaine (25%); and none for the 6 opiates positive cases and 3 MDMA cases, respectively.

Conclusion

Drugs/metabolites in hair of pregnant women can be used as biomarkers of past drug use (repetitive or sporadic), although the use is not always reflected in fetal/placental tissues. There are several possible hypotheses to explain the results: (1) the use occurred before the start of pregnancy, (2) past sporadic consumption which could be measured in hair but not in fetal and placental remains because of the narrow window of drug detection in placental/fetal tissues; (3) the sensitivity of the analytical methods was not high enough for the detection of the minute amount of drugs of abuse and metabolites which reached these tissues (4) there is a large variability in the transplacental passage of drugs of abuse and in the placenta's metabolizing capacity.