A peptide with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Russula paludosa
- 作者：Jianbin Wang ; H.X. Wang ; T.B. Ng
- 关键词：AIDS ; HIV ; Reverse transcriptase (RT) ; Purification ; Edible and medicinal mushrooms
- 刊名：Peptides
- 出版年：2007
- 期刊代码：59_01969781
- 类别：neu
- 出版时间：March 2007
- 卷：28
- 期：3
- 页码：560-565
- 文件大小：296 K
摘要
Hot water extracts of 16 species of mushrooms, including both edible and medicinal mushrooms, were screened for human immunodeficiency virus (HIV)-1 reverse transcriptase (RT) inhibitory activity. Extracts of Lactarius camphoratus, Trametes suaveolens, Sparassis crispa, Pleurotus sajor-caju, Pleurotus pulmonarius, and Russula paludosa elicited over 50%inhibition when tested at the concentration of 1 mg/ml. The extract of R. paludosa demonstrated the highest inhibitory activity on HIV-1 RT (97.6%). Fraction SU2, purified from R. paludosa extract by anion exchange chromatography on DEAE-cellulose and gel filtration on Superdex 75, exhibited potent inhibitory activity on HIV-1 RT. At the concentrations of 1 mg/ml, 0.2 mg/ml, and 0.04 mg/ml, the inhibition ratios were 99.2%, 89.3%, and 41.8%, respectively, giving an IC50 of 11 μM. The molecular mass of SU2 was 4.5 kDa and its N-terminal amino acid sequence was determined to be KREHGQHCEF. The peptide was devoid of hemagglutinating, ribonuclease, antifungal, protease, protease inhibitory, and laccase activities.