A novel water-soluble 尾-d-glucan isolated from the spores of Ganoderma lucidum

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• 作者：Qun Dong^a ; ^{qdong@mail.shcnc.ac.cn} ; Ying Wang^a ; Lei Shi^a ; Jian Yao^a ; Jie Li^a ; Fangli Ma^b ; Kan Ding^a ; ^{kding@mail.shcnc.ac.cn}
• 关键词：Ganoderma lucidum ; 尾-(1&rarr ; 6)-Glucan ; Polysaccharide ; Structure
• 刊名：Carbohydrate Research
• 出版年：2012
• 期刊代码：15_00086215
• 类别：ch
• 出版时间：15 May, 2012
• 卷：353
• 期：Complete
• 页码：100-105
• 文件大小：645 K

摘要

Ganoderma lucidum is an edible and medicinal mushroom used widely in East Asia. In recent years, its spores have been used as a supplement in combination with other forms of antitumor therapies. The cell wall of Ganoderma lucidum spores contains a high amount of polysaccharides. In this study, a neutral polysaccharide, GLSA50-1B, was isolated from sporoderm-broken spores of Ganoderma lucidum, by hot-water extraction, graded ethanol precipitation, anion-exchange chromatography, and gel permeation chromatography. Using sugar compositional analysis, methylation analysis, partial acid hydrolysis, acetolysis, and NMR and ESI-MS spectroscopy, GLSA50-1B was elucidated to be a novel 尾-d-glucan featured by a 1,6-linked 尾-d-Glcp backbone with different length of branches consisting of terminal and 1,4-linked Glcp residues, attached to O-4 of alternative Glc residues in the backbone.