Effects of extraction methods on the composition and molar mass distributions of exopolymeric substances of the bacterium Sinorhizobium meliloti

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• 作者：Enrica Alasonati^a ; ¹ ; Vera I. Slaveykova^a ; ^b ; ^{vera.slaveykova@unige.ch}
• 关键词：EPS ; extracellular polymeric substances ; WT1 ; soluble EPS isolated from wild type Sinorhizobium meliloti ; WT2 ; EPS of wild type bacteria extracted by EDTA ; WT3 ; EPS of wild type bacteria extracted by formaldehyde/NaOH ; EXOy1 ; soluble EPS isolated from exo
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：June, 2012
• 卷：114
• 期：Complete
• 页码：603-609
• 文件大小：604 K

摘要

The influence of the extraction methods on the composition, size diversity, molar mass and size distributions of exopolymeric substances (EPS) from the bacterium Sinorhizobium meliloti wild type (WT) and by the exoY strain deficient in exopolysaccharide production was investigated. EPS obtained by centrifugation, EDTA and formaldehyde/NaOH were compared. It was found that the extraction method influenced TOC, TN and total protein content in EPS from both strains. However, no difference between EDTA and formaldehyde/NaOH methods was observed for the exopolysaccharide components. Similar functional groups and fluorescence pattern were found in the EPS obtained by different methods; however their relative abundance was method dependent. The extraction method also affected the molar mass and size distribution, HP SEC diversity among different treatment and bacterial strains.