Electrochemical Detector Based on Nafion Membrane for Online Monitoring of Column Chromatography Purification Process

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• 作者：Yu-Hang TANG^a ; Wen-Han LIU^a ; ^{liuwh@zjut.edu.cn} ; Yuan-Jie TENG^a ; Jun-Qing QIAN^b ; Chun-An MA^a
• 关键词：Natural medicine ; Nafion membrane ; Antioxidant of bamboo leaves ; Cyclic Voltammetry ; Micro-gap flow Cell ; Paraffin-graphite Powder electrode
• 刊名：Chinese Journal of Analytical Chemistry
• 出版年：2012
• 期刊代码：4_18722040
• 类别：ch
• 出版时间：May, 2012
• 卷：40
• 期：5
• 页码：675-680
• 文件大小：308 K

摘要

Electrochemical detection of eluent of column chromatography is considerably simple for monitoring the purification process, In this detection system, electrolyte is needed to decrease the resistance of solution during the electrochemical detection. However, in the case of natural medicine purification, it is highly challenging to added electrolyte into the purification process without influencing the purification efficiency. Herein, on the basis of Paraffin-graphite Powder electrode, an online electrochemical detector was developed without any additional electrolyte. The cyclic voltammograms of myricetin in ethanol-water (80%, V/V) as mobile phase is obtained directly by using the solid acid characteristic of Nafion N-324 membrane in the micro-gap flow cell. Peristaltic pump stimulates the chromatographic process, and then it realizes the online detection of eletroactive substances in the eluent at 0.53 V. Peak area is linear with the myricetin concentration in the range of 2.0 脳 10^鈭?鈭?.0 脳 10^鈭? M, and the linear equation is A = 2.7227c + 0.4341, r = 0.9976. Furthermore, the detector achieves the online detection of 30%AOB (antioxidant of bamboo leaves) purified by macro porous resin without any supporting electrolyte in 80%(V/V) ethanol-water at 0.62 V, and the amperometric time-current (t-i) curve during the purification process was presented. The result indicated that the detector is feasible for application.