Characterization of ultra-high molar mass hyaluronan: 1. Off-line static methods
- 作者：Mendichi ; Raniero ; Schieroni ; Alberto Giacometti ; Grassi ; Cesare ; Re ; Alberto
- 关键词：hyaluronan ; ultra-high molar mass ; multi-angle light scattering
- 刊名：Polymer
- 出版年：1998
- 期刊代码：83_00323861
- 类别：ms
- 出版时间：December, 1998
- 卷：39
- 期：25
- 页码：6611-6620
- 文件大小：587 K
摘要
This study concerns the problems encountered in the molecular characterization of ultra-high molar mass (UHMM) hyaluronan samples. The determination of the molar mass distribution of UHMM polymers is fairly difficult. Shear degradation and non-newtonian flow are the main difficulties in the fractionation on SEC columns. Considering these difficulties, the samples have remained previously characterized by off-line light scattering and viscometry. The characterization by means of light scattering presents the problem of non linear angular variation of the scattering. We present a method to estimate reliable values for molar mass and dimensions of UHMM hyaluronan polymers. To overcome the intrinsic viscosity shear rate dependence we have used a multi-bulb capillary viscometer. The persistence length of the UHMM hyaluronan polymer has been estimated.