Isolation and characterization of a muskmelon cDNA encoding Lycopene Beta-cyclase

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• 作者：Hui Hao¹ ; Leyuan Ma¹ ; Hongzi Cong ; Qiang Li ; Xiyan Yu ; ^{yuxiyan@sdau.edu.cn}
• 关键词：Bp ; base pair(s) ; cDNA ; DNA complementary to RNA ; cv. ; cultivar ; DAP ; day after pollination ; DNA ; deoxyribonucleic acid ; HPLC ; high-performance liquid chromatography ; mRNA ; messenger RNA ; PCR ; polymerase chain reaction ; RT-PCR ; reverse transcription PCR
• 刊名：Gene
• 出版年：2012
• 期刊代码：73_03781119
• 类别：bio
• 出版时间：15 July, 2012
• 卷：503
• 期：1
• 页码：147-151
• 文件大小：807 K

摘要

Lycopene Beta-cyclase (LCY-B) is thought to play a critical role in Beta鈥恈arotene synthesis in fruit. A full-length cDNA clone encoding Lycopene Beta-cyclase was isolated from muskmelon (Cucumis melo L.) by RT-PCR and RACE. The clone, designated CmLcyb1, contains 1871 nucleotides, with an open reading frame of 1512 nucleotides. The deduced 504-amino-acid sequence showed high identities with other plant Lycopene Beta-cyclases. Real time quantitative RT-PCR analysis indicated that CmLcyb1 was expressed in all tissues and organs of muskmelon inbred M01-3 with white mesocarp and, 鈥楬omoka鈥? an orange mesocarp cultivar. The expression levels of CmLcyb1 in roots, stems, leaves and flowers in the two genotypes differed little. The expression level was highest in mature fruit of 鈥楬omoka鈥?and was much higher than that in mature fruit of M01-3. Moreover, the mRNA level of CmLcyb1 was very low in fruits before fruit-size fixation and increased dramatically in the size-fixed fruits of these two genotypes. The mRNA levels of CmLcyb1 during fruit development of 鈥楬omoka鈥?were all higher than those of M01-3. Interestingly, Beta鈥恈arotene content showed almost the same change trend as mRNA levels during fruit development in these two genotypes, suggesting that Beta鈥恈arotene accumulation may be linked to the CmLcyb1 transcript level in muskmelon fruit.