Chemical and chromatographic stability of methacrylate-based monolithic columns
- 作者：Jana Vidič ; Aleš ; Podgornik ; Janez Janč ; ar ; Vida Frankovič ; Boš ; tjan Koš ; ir ; Nika Lendero ; Karmen Č ; uč ; ek ; Matjaž ; Krajnc ; Aleš ; Š ; trancar
- 关键词：Chemical stability ; Chromatographic stability ; Mechanical stability ; Methacrylate monoliths ; Anion-exchange columns ; Ligand leakage ; CIM
- 刊名：Journal of Chromatography A
- 出版年：2007
- 期刊代码：47_00219673
- 类别：ch
- 出版时间：9 March 2007
- 卷：1144
- 期：1
- 页码：63-71
- 文件大小：217 K
摘要
Chemical and chromatographic stability of methacrylate-based monolithic columns bearing 3-N,N-diethylamino-2-hydroxypropyl (DEAE) and quarternary amine (QA) groups was studied. The leakage products from both monolithic columns were determined and the leakage of amines has been quantified in alkali solutions. Monolithic columns bearing QA functional groups being exposed to 1 M sodium hydroxide solution for up to 3 months caused reduction of ion-exchange groups for approximately 12%, while for DEAE monolithic columns was only around 3%in 1 year. In 0.1 M NaOH and 20%ethanol degradation was significantly lower. The main leaking compound from DEAE monolith was found to be 3-(diethylamino)-1,2-propanediol and 2,3-dihydroxypropyltrimethylammonium salt for QA monolith. During repeated 50 cleaning-in-place (CIP) cycles, no changes in chromatographic properties were detected.