Macroporous methacrylate-based monoliths as platforms for DNA microarrays

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• 作者：Ekaterina S. Sinitsyna^a ; Johanna G. Walter^b ; Evgenia G. Vlakh^a ; Frank Stahl^b ; Cornelia Kasper^b ; Tatiana B. Tennikova^a ; ^c ; ^{tennikova@mail.ru}
• 关键词：GMA ; glycidyl methacrylate ; EDMA ; ethylene dimethacrylate ; HEMA ; 2-hydroxyethyl methacrylate ; CEMA ; 2-cyanoethyl methacrylate ; GDMA ; glycerol dimethacrylate ; 2-D and 3-D ; two and three-dimensional ; respectively ; DMAP ; 4-(dimethylamino)pyridine ; CDI ; 1 ; 1-c
• 刊名：Talanta
• 出版年：2012
• 期刊代码：106_00399140
• 类别：ch
• 出版时间：15 May, 2012
• 卷：93
• 期：Complete
• 页码：139-146
• 文件大小：685 K

摘要

Macroporous monoliths with different surface functionalization (reactive groups) were utilized as platforms for DNA analysis in microarray format. The slides based on a copolymer glycidyl methacrylate-co-ethylene dimethacrylate (GMA-EDMA) have been chosen as well known and thoroughly studied standard. In particular, this material has been used at optimization of DNA microanalytical procedure. The concentration and pH of spotting solution, immobilization temperature and time, blocking agent and coupling reaction duration were selected as varied parameters. The efficiency of analysis performed on 3-D monolithic platforms was compared to that established for commercially available glass slides. As a practical example, a diagnostic test for detection of CFTR gene mutation was carried out. Additionally, the part of presented work was devoted to preparation of aptamer-based test-system that allowed successful and highly sensitive detection both of DNA and protein.