Separation of small inorganic anions using methacrylate-based anion-exchange monolithic column prepared by low temperature UV photo-polymerization

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• 作者：Mutsumi Takahashi ; Tomohiko Hirano ; Shinya Kitagawa ; ^{kitagawa.shinya@nitech.ac.jp} ; Hajime Ohtani
• 关键词：Anion-exchange ; Methacrylate-based monolith ; Inorganic anions ; Low temperature photo-polymerization ; Fast separation
• 刊名：Journal of Chromatography A
• 出版年：2012
• 期刊代码：47_00219673
• 类别：ch
• 出版时间：6 April, 2012
• 卷：1232
• 期：Complete
• 页码：123-127
• 文件大小：544 K

摘要

A methacrylate-based anion-exchange monolithic column was prepared by a single-step UV photo-copolymerization of [2-(methacryloyloxy)ethyl]-trimethyl ammonium chloride, butylmethacrylate, and ethylene dimethacrylate at a low temperature of 鈭?5 掳C. The anion-exchange column exhibited good separation efficiency for the small inorganic anions of NO₂^{鈭?/sup>, Br鈭?/sup>, NO₃鈭?/sup>, and I鈭?/sup> in the isocratic mode. Under the conditions optimized using a mobile phase of 50%ACN containing 100 mM of NaCl, the theoretical plate heights of the anions were within the range of 12.2-15.6 渭m (N, 64,000-82,000 m鈭?; k, 0.2-1.6); and a value of 9.4 渭m (N, 110,000 m鈭?) was achieved for t₀. The flow resistance of the column was acceptably low with a permeability of 2.7 脳 10鈭?3 m2. Fast gradient elution at a flow rate of 32 mm/s resulted in rapid and precise separation of the inorganic anions of IO₃鈭?/sup>, NO₂鈭?/sup>, Br鈭?/sup>, NO₃鈭?/sup>, and I鈭?/sup> within 20 s.}