Azlactone-reactive polymer supports for immobilizing synthetically useful enzymes. II. Important preliminary hydrogen bonding effects in the covalent coupling of Penicillin G Acylase
- 作者：Gary J. Drtina ; Louis C. Haddad ; Jerald K. Rasmussen ; Babu N. Gaddam ; Michael G. Williams ; Stephanie J. Moeller ; Robert T. Fitzsimons ; Duane D. Fansler ; Tara L. Buhl ; Yeng N. Yang et al.
- 关键词：Penicillin G Acylase ; E.C. 3.5.1.11 ; Azlactone ; Emphaze™ ; AB1 ; Immobilized enzyme ; Biocatalysis
- 刊名：Reactive and Functional Polymers
- 出版年：2005
- 期刊代码：92_13815148
- 类别：ch
- 出版时间：2005
- 卷：64
- 期：1
- 页码：13-24
- 文件大小：492 K
摘要
Azlactone-functional dispersion and reverse phase suspension polymer supports were examined as immobilizing media for Penicillin G Acylase (PGA). Results indicated that the most effective supports were those that also contained primary and/or secondary amide functional groups. A combination of hydrophobic interactions and hydrogen bonding between amide groups on the support and enzyme was proposed to provide important and intimate association between PGA and support prior to covalent coupling. Relative to an oxirane-functional commercial standard, the azlactone biocatalyst supports featured shorter coupling times and higher catalytic activities.