Sample introduction techniques for microchip electrophoresis: A review
- 作者：James M. Karlinsey ; jmk48@psu.edu ; [Author Vitae]
- 关键词：CE ; capillary electrophoresis ; CGE ; capillary gel electrophoresis ; CZE ; capillary zone electrophoresis ; EOF ; electroosmotic flow ; IDEA ; integrated dielectric elastomer actuator ; ME ; microchip electrophoresis ; MGE ; microchip gel electrophoresis ; 渭TAS ; mic
- 刊名：Analytica Chimica Acta
- 出版年：2012
- 期刊代码：2_00032670
- 类别：ch
- 出版时间：6 May, 2012
- 卷：725
- 期：Complete
- 页码：1-13
- 文件大小：1714 K
摘要
The number of applications of microfluidic analysis systems continues to increase, along with the variety of substrate materials and complexity of the devices themselves. One of the most common features of these devices that has remained relatively unchanged, however, is the introduction of a sample mixture into a separation channel so that individual components can be separated by electrophoresis. Whether a relatively simple mixture of amino acids or a more complex sample of DNA fragments extracted and amplified on-chip, the ability to reliably and reproducibly inject a representative sample is arguably the most significant requirement for an electrophoretic micro total analysis system (渭TAS). This review will focus on the different methods reported for sample introduction in microchip electrophoresis, highlighting both pressure-driven and electrokinetic techniques, with an emphasis on the methods employed in 渭TAS applications.