An amperometric glucose biosensor prototype fabricated by thermal inkjet printing
- 作者：Setti ; L. ; Fraleoni-Morgera ; A. ; Ballarin ; B. ; Filippini ; A. ; Frascaro ; D. ; Piana ; C.
- 关键词：Thermal inkjet printing ; Biological ink ; Electronic ink ; Bioelectronics ; Biopolytronics ; Biosensors
- 刊名：Biosensors and Bioelectronics
- 出版年：2005
- 期刊代码：12_09565663
- 类别：ch
- 出版时间：April 15, 2005
- 卷：20
- 期：10
- 页码：2019-2026
- 文件大小：329 K
摘要
The prototype of an amperometric glucose biosensor was realized by thermal inkjet printing using biological and electronic water-based inks, containing a glucose oxidase (GOD) from Aspergillus niger and the conducting polymer blend poly(3,4-ethylenedioxythiophene/polystyrene sulfonic acid) (PEDOT/PSS), respectively. The biosensor was fabricated microdepositing PEDOT/PSS and GOD, in sequence, on ITO-glass, by a commercial inkjet printer, with the help of a commercial software. High density microdots matrices were so-realized, with a calculated resolution of about 221×221dpi (dot per inch). By means of a rapid and easy assay it was demonstrated that no activity loss occurred upon the printing of GOD, despite of the use of a thermal printhead. The device was encapsulated in a semipermeable membrane of cellulose acetate, applied by dip-coating, in order to prevent dissolution of the enzyme and/or PEDOT/PSS in water. The preliminary response of the electrode was measured in an aqueous glucose solution in the presence of ferrocenemethanol (FeMeOH) as a mediator, and resulted linear up to 60mM in glucose. The best sensitivity value achieved was 6.43μAM−1cm−2 (447nAM−1U−1cm−2). The characteristics of the device, and the possible performance improvements have been analyzed and discussed. The reported findings indicate that inkjet printing could be a viable instrument for the easy construction of a working biosensor via direct digital design using biological and conductive polymer based inks. Such an approach may be seen as an example of “biopolytronics”.