Genotoxicity studies on green tea catechin
- 作者：R. Ogura ; N. Ikeda ; K. Yuki ; O. Morita ; K. Saigo ; C. Blackstock ; N. Nishiyama ; T. Kasamatsu
- 关键词：Catechin ; Genotoxicity ; Green Tea ; Beverage
- 刊名：Food and Chemical Toxicology
- 出版年：2008
- 期刊代码：20_02786915
- 类别：pha
- 出版时间：June 2008
- 卷：46
- 期：6
- 页码：2190-2200
- 文件大小：182 K
摘要
The beneficial effects of tea catechins are well documented. We evaluated the genotoxic potential of a green tea catechin preparation using established genotoxicity assays, including a bacterial reverse mutation assay (Ames test), a chromosomal aberration assay in cultured Chinese hamster lung cells (CHL/IU), a mouse lymphoma L5178Y/tk assay, and a bone marrow micronucleus (MN) assay in ICR CD mice and SD rats. No significant increases in the number of revertant colonies were observed in the Ames test, but positive responses were observed in two in vitro assays: the chromosomal aberration assay and mouse lymphoma L5178/tk assay. However, the in vivo study demonstrated no significant increase in micronucleated polychromatic erythrocytes (MNPCE) in the bone marrow of both ICR CD mice and SD rats administered a high dose of the green tea catechin preparation up to 2000 mg/kg. Combined with favorable epidemiological information suggesting a chemopreventive effect of tea catechins on carcinogenesis, we conclude that green tea catechin presents no significant genotoxic concern under the anticipated conditions of use. These results are consistent with other genotoxicity studies of tea catechins, which show minimal, if any, genotoxic potential.