摘要
Pinus henryi, a rare and endemic pine in China, has been misidentified as its widespread congener Pinus tabuliformis. In this study, ten nuclear microsatellite markers were employed to determine the population genetic structure and geographic differentiation of the two species. P. henryi and P. tabuliformis exhibited both a high level of genetic diversity (He聽=聽0.8917) and distinct genetic differentiation (Fst聽=聽0.3511) among populations. This could be attributed to a complex and fragmented habitat, historical bottleneck effect, adaptation to environments, and forced inbreeding. A neighbor-joining tree based on a genetic distance algorithm revealed that the boundary between the two pines lay between the Qing-Daba Mountains and their divergence was possibly caused by habitat differences. Although little historical gene flow occurred among populations, a recent unilateral individual migration of P. henryi to the P. tabuliformis areas probably occurred because of expansion under the background of global warming.