Importance of elastase production in development of invasive aspergillosis

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• 作者：A.R. Khosravi^a ; ^{Khosravi@ut.ac.ir} ; S. Mahdavi Omran^b ; H. Shokri^c ; A. Lotfi^d ; Z. Moosavi^e
• 关键词：Aspergillus fumigatus ; Aspergillosis ; Elastase ; Pathogenicity
• 刊名：Journal de Mycologie Mé ; dicale / Journal of Medical Mycology
• 出版年：2012
• 期刊代码：156_11565233
• 类别：med
• 出版时间：June, 2012
• 卷：22
• 期：2
• 页码：167-172
• 文件大小：425 K

摘要

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Summary

Objective

To evaluate the relationship between elastase activity of different Aspergillus fumigatus (A.聽fumigatus) isolates and their pathogenicity in mice.

Material and methods

The animals were intravenously (IV) infected with 1 脳 10⁶ conidia/mouse. The elastase activity in mice sera and survival time were determined. All animals were sacrified at the end of the fourth week, and autopsies were performed for histopathological examination. The tissue sections were stained with Periodic Acid Schiff (PAS) and Verhoeff's techniques.

Results

The highest elastase activity was associated with the sera of infected mice with elastase-producing A.聽fumigatus AIR78 as standard strain (0.18 卤 0.02 mm), followed by infected animals with elastase-producing isolates (0.16 卤 0.02 mm) and control mice (0.14 卤 0.02 mm). The elastase activity index (EAI) was determined in value of 0.92 卤 0.27. The survival times in mice infected with elastase-producing isolate and in control group were 21.6 and 30 days, respectively. Histopathological studies showed that the heart and kidneys were more infected by Aspergillus hyphae than other tissues.

Conclusion

It was concluded that there is a significant relationship between elastase activity and survival time in mice with aspergillosis.