Usefulness of Chromogenic CromoCen庐 AGN agar medium for the identification of the genus Aeromonas: Assessment of faecal samples

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• 作者：M.G. Aguilera-Arreola^a ; M.I. Portillo-Muñ ; oz^a ; C. Rodrí ; guez-Martí ; nez^b ; G. Castro-Escarpulli^a ; ^{chelacastro@hotmail.com}
• 关键词：Aeromonas isolation ; Chromogenic medium ; Ecometric technique
• 刊名：Journal of Microbiological Methods
• 出版年：2012
• 期刊代码：59_01677012
• 类别：imm
• 出版时间：August, 2012
• 卷：90
• 期：2
• 页码：100-104
• 文件大小：182 K

摘要

Selective screening media for the detection and identification of Aeromonas strains are needed to guide primary isolation procedures in the clinical laboratory. This study compared the selective CromoCen庐 AGN chromogenic agar medium for the detection and identification of Aeromonas strains that were isolated from various samples against the conventional selective agar media that are commonly used for the isolation of this organism in food, environmental and clinical samples. The Miles and Misra and ecometric methods were used to evaluate the microbiological performance of CromoCen庐 AGN chromogenic agar medium, which was shown to be satisfactory. A total of 14 reference Aeromonas strains, 44 wild strains and 106 clinical stool specimens were examined using both non-chromogenic selective agars that are commonly used for Aeromonas isolation and CromoCen庐 AGN agar. The latter exhibited 94.73%sensitivity and 100%specificity for the various samples. On CromoCen庐 AGN agar medium, Aeromonas formed colonies with light green, greenish and salmon pigments with or without a surrounding wide transparent zone (halo) of 2-3 mm in diameter around the entire border. This medium is recommended for the isolation and potential identification of the Aeromonas genus.