LAL activity in DBS extracts was measured using the substrate 4-methylumbelliferyl palmitate. LAL activity was determined by measuring total lipase activity and lipase activity in the presence of Lalistat 2. The specificity of Lalistat 2 was investigated using human recombinant LAL (hrLAL) and human pancreatic lipase (hPL).<h4 class="h4">Resultsh4>
Lalistat 2 inhibited hrLAL with 1%residual activity at 1 渭M inhibitor but had no effect on hPL up to 10 渭M. LAL activity in DBS samples obtained from normal controls (n = 140) was 0.50-2.30 nmol/punch/h and in patients with CESD was < 0.03 nmol/punch/h (n = 11). Activity in carriers showed intermediate activity: 0.15-0.40 nmol/punch/h (n = 15).<h4 class="h4">Conclusionsh4>
Measurement of LAL using DBS is made difficult by the presence of other lipases in whole blood. Lalistat 2 is a specific inhibitor of LAL which allows the determination of LAL in DBS. Results show the method differentiates clearly between normal controls, carriers and affected cases.