The stimulation of adipose-derived stem cell differentiation and mineralization by ordered rod-like fluorapatite coatings

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• 作者：Jun Liu^a ; ^{junlc@umich.edu} ; ^{junl999@yahoo.com} ; Xiaodong Wang^a ; ^b ; Qiming Jin^c ; Taocong Jin^a ; Syweren Chang^a ; Zhaocheng Zhang^a ; Agata Czajka-Jakubowska^d ; William V. Giannobile^c ; Jacques E. Nö ; r^a ; Brian H. Clarkson^a
• 关键词：Fluorapatite ; Differentiation ; Mineralization ; Gene profile ; Stem cells
• 刊名：Biomaterials
• 出版年：2012
• 期刊代码：7_01429612
• 类别：ms
• 出版时间：July, 2012
• 卷：33
• 期：20
• 页码：5036-5046
• 文件大小：1550 K

摘要

In this study, the effect of ordered rod-like FA coatings of metal discs on adipose-derived stem cell (ASC)鈥檚 growth, differentiation and mineralization was studied in聽vitro; and their mineral inductive effects in聽vivo. After 3 and 7 days, the cell number on the metal surfaces was significantly higher than those on the ordered and disordered FA surfaces. However, after 4 weeks much greater amounts of mineral formation was induced on the two FA surfaces with and even without osteogenesis induction. The osteogenic profiles showed the up regulation of a set of pro-osteogenic transcripts and bone mineralization phenotypic markers when the ASCs were grown on FA surfaces compared to metal surfaces at 7 and 21 days. In addition to BMP and TGF尾 signaling pathways, EGF and FGF pathways also appeared to be involved in ASC differentiation and mineralization. In聽vivo studies showed accelerated and enhanced mineralized tissue formation integrated within ordered FA coatings. After 5 weeks, over 80%of the ordered FA coating was integrated with a mineralized tissue layer covering the implants. Both the intrinsic properties of the FA crystals and the topography of the FA coating appeared to dominate the cell differentiation and mineralization process.