Immobilization of Candida rugosa lipase on hydrophobic/strong cation-exchange functional silica particles for biocatalytic synthesis of phytosterol esters

详细信息	查看全文 | 推荐本文 |

• 作者：Ming-Ming Zheng^a ; Yong Lu^b ; Ling Dong^a ; Ping-Mei Guo^a ; Qian-Chun Deng^a ; Wen-Lin Li^a ; Yu-Qi Feng^b ; Feng-Hong Huang^a ; ^{mingmingabc1983@163.com}
• 关键词：Immobilized lipase ; Mixed-mode silica particles ; Hydrophobic interaction/cation-exchange ; Biocatalysis ; Esterification
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：July, 2012
• 卷：115
• 期：Complete
• 页码：141-146
• 文件大小：321 K

摘要

In this work, mixed-mode silica particles functionalized with octyl and sulfonic acid groups was conveniently prepared by co-bonding a mixture of n-octyltriethoxysilane and 3-mercaptopropyltriethoxysilane and then oxidized with hydrogen peroxide. Candida rugosa lipase (CRL) was immobilized on the mixed-mode silica particles via hydrophobic and strong cation-exchange interaction. The resulting immobilized CRL increased remarkably its stability at high temperature in comparison to free CRL. The immobilized CRL was used as biocatalysts for enzymatic esterification of phytosterols with free fatty acids (FFAs) to produce phytosterol esters. The phytosterols linolenate esterification degree of 95.3%was obtained under the optimized condition. Phytosterols esters could also been converted in high yields to the corresponding long-chain acyl esters via transesterification with methyl esters of fatty acids (80.5%) or triacylglycerols (above 95.5%) using mixed-mode silica particles immobilized CRL as biocatalyst. Furthermore, the immobilized CRL by absorption retained 78.6%of their initial activity after 7 recycles.