ZNF307, a novel zinc finger gene suppresses p53 and p21 pathway
- 作者：Jing Li ; Yuequn Wang ; Xiongwei Fan ; Xiaoyang Mo ; Zequn Wang ; Yongqing Li ; Zhaochu Yin ; Yun Deng ; Na Luo ; Chuanbing Zhu ; Mingyao Liu ; Qian Ma ; Karen Ocorr ; Wuzhou Yuan ; Xiushan Wu
- 关键词：KRAB ; ZNF307 ; p53 ; p21
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：30 November 2007
- 卷：363
- 期：4
- 页码：895-900
- 文件大小：1227 K
摘要
We have cloned a novel KRAB-related zinc finger gene, ZNF307, encoding a protein of 545 aa. ZNF307 is conserved across species in evolution and is differentially expressed in human adult and fetal tissues. The fusion protein of EGFP-ZNF307 localizes in the nucleus. Transcriptional activity assays show ZNF307 suppresses transcriptional activity of L8G5-luciferase. Overexpressing ZNF307 in different cell lines also inhibits the transcriptional activities of p53 and p21. Moreover, ZNF307 works by reducing the p53 protein level and p53 protein reduction is achieved by increasing transcription of MDM2 and EP300. ZNF307 might suppress p53–p21 pathway through activating MDM2 and EP300 expression and inducing p53 degradation.