The content of individual minor alkaloids, which was obtained was relatively low (0.00-0.17%) and in some cases negligible, defined by the dominant representation of morphine in the alkaloid spectrum of all selected cultivars. In addition to the high-morphine cultivar 鈥楤uddha鈥?(mean 1.64%), there was a middle content (mean 0.79%) or low content (mean 0.61%) of morphine in the cultivars included in clusters 2 and 3 (average of 3 consecutive years 2007, 2008 and 2009). A relatively high content of morphine was detected in cultivar 鈥楤枚hm暖v b臎losemenn媒鈥?(mean 0.91%), although it is usually published that white-seed cultivars contain less morphine and other alkaloids, compared to blue-seed or grey-seed cultivars. Low content of morphine (mean 0.48%resp. 0.44%) by relatively high content of narcotine (mean 0.11%resp. 0.15%) was obtained in poppy cultivars 鈥楧anneborg鈥?and 鈥楾atarstan鈥? Also, surprisingly the highest papaverine content (mean 0.07%) was detected in the cultivar 鈥楽u拧ick媒 膷ervenosemenn媒鈥?with the lowest content of morphine (mean 0.34%).