Diverse patterns of poly(A) tail elongation and shortening of murine maternal mRNAs from fully grown oocyte to 2-cell embryo stages
- 作者：Takayuki Sakurai ; Masahiro Sato and Minoru Kimura
- 关键词：Mouse ; Oocyte ; Fertilized egg ; Preimplantation embryo ; Maternal RNA ; Translational control ; Poly(A) tail ; Cytoplasmic polyadenylation ; Deadenylation ; cDNA library
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2005
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：2005
- 卷：336
- 期：4
- 页码：1181-1189
- 文件大小：521 K
摘要
We previously succeeded in constructing a cDNA library, CPF7, enriched with cDNA derived from maternal RNAs with the extended poly(A) tail in mouse fertilized eggs. In this study, we performed RNA blot analysis to examine the elongation in maternal RNAs using 20 representative clones isolated from CPF7 as probes. Various patterns of elongation, shortening, and/or degradation of maternal RNAs were observed from fully grown oocytes to early 2-cell embryos and could be roughly classified into three types and seven subtypes. These findings indicate that poly(A) elongation and shortening of maternal RNAs are not restricted to certain types of maternal RNAs but occur in many of them, and suggest a complex mechanism governing modification of the 3′ end of maternal RNAs during the oocyte-to-embryo transition.