Influence of serotype on the growth kinetics and the ability to form biofilms of Salmonella isolates from poultry

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• 作者：Miryam D&iacute ; ez-Garc&iacute ; a ; Rosa Capita ; Carlos Alonso-Calleja ; ^{carlos.alonso.calleja@unileon.es}
• 关键词：Salmonella serotypes ; Poultry ; Biofilms ; Growth kinetics
• 刊名：Food Microbiology
• 出版年：2012
• 期刊代码：100_07400020
• 类别：imm
• 出版时间：September, 2012
• 卷：31
• 期：2
• 页码：173-180
• 文件大小：492 K

摘要

The influence of the serotype on the growth behaviour and the ability to form biofilms of Salmonella enterica strains was investigated. The relationships between biofilm formation and growth kinetic parameters were also determined. A total of 69 strains (61 isolates from poultry and 8 reference strains from culture collections) belonging to 10 serotypes (S.聽enterica serotype Typhimurium, S. Newport, S. Paratyphi B, S. Poona, S. Derby, S. Infantis, S. Enteritidis, S. Virchow, S. Agona and S. Typhi) were tested. All Salmonella strains produced biofilms on polystyrene micro-well plates (crystal violet assay). Isolates were classified as weak (35 strains), moderate (22), or strong (12) biofilm producers. S. Agona and S. Typhi produced the most substantial (P聽<聽0.001) biofilms. Growth curves were performed at 37聽掳C in tryptone soy broth by means of optical density (OD_420-580) measurements from 0 to 48聽h. Growth kinetic parameters (Gompertz model) varied between serotypes. The maximum growth rate (螖OD_420-580/h) ranged from 0.030聽卤聽0.002 (S. Typhi) to 0.114聽卤聽0.011 (S. Agona). The ability of Salmonella strains to form biofilms was not related to their growth kinetic parameters. The formation of biofilms by Salmonella on polystyrene constitutes an issue of concern because plastic materials are frequently used in food facilities. The findings suggest that special efforts must be made for the effective control of Salmonella in food-processing environments when S. Agona or S. Typhi strains are present.