Isohumulones modulate blood lipid status through the activation of PPARα
- 作者：Masako Shimura ; Akiko Hasumi ; Toshiko Minato ; Mayu Hosono ; Yutaka Miura ; Satoru Mizutani ; Keiji Kondo ; Shinichi Oikawa and Aruto Yoshida
- 关键词：Isohumulone ; Hop ; Beer ; PPARα ; Fatty acid oxidation ; Triglyceride ; Liver ; Microarray ; Nutrigenomics ; Atherosclerosis
- 刊名：Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids
- 出版年：2005
- 期刊代码：18_13881981
- 类别：bio
- 出版时间：2005
- 卷：1736
- 期：1
- 页码：51-60
- 文件大小：359 K
摘要
Isohumulones derived from hops are the major bitter compounds in beer. It was recently reported that isohumulones activated peroxisome proliferator-activated receptors (PPARs) α and γ in vitro and modulated glucose and lipid metabolism in vivo. In this study, we examined the effects of isomerized hop extract (IHE) primarily containing isohumulones in C57BL/6N male mice and found that such treatment increased their liver weight and reduced their plasma triglyceride and free fatty acid levels. Microarray analysis and quantitative real time PCR (QPCR) showed that IHE dose-dependently upregulated the expression of a battery of hepatic genes that are involved in microsomal ω-oxidation and peroxisomal and mitochondrial β-oxidation. These effects were common in both genders and very similar to those found with the PPARα agonist, fenofibrate (FF). Moreover, these effects were not found in PPARα-deficient mice. Thus, our results strongly suggest that IHE intake upregulates the expression of key genes that are involved in hepatic fatty acid oxidation, and that it ameliorates the blood lipid profile by activating PPARα.