Cytokinesis is not controlled by calmodulin or myosin light chain kinase in the Caenorhabditis elegans early embryo

详细信息	查看全文 | 推荐本文 |

• 作者：Ellen L. Batchelder ; Christina L. Thomas-Virnig ; Jeffery D. Hardin ; John G. White
• 关键词：Calmodulin ; Cytokinesis ; MLCK ; Phosphorylation ; Caenorhabditis elegans
• 刊名：FEBS Letters
• 出版年：2007
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：4 September 2007
• 卷：581
• 期：22
• 页码：4337-4341
• 文件大小：257 K

摘要

Furrow ingression in animal cell cytokinesis is controlled by phosphorylation of myosin II regulatory light chain (mRLC). In Caenorhabditis elegans embryos, Rho-dependent Kinase (RhoK) is involved in, but not absolutely required for, this phosphorylation. The calmodulin effector myosin light chain kinase (MLCK) can also phosphorylate mRLC and is widely regarded as a candidate for redundant function with RhoK. However, our results show that RNA mediated interference against C. elegans calmodulin and candidate MLCKs had no effect on cytokinesis in wild-type or RhoK mutant embryos, ruling out the calmodulin/MLCK pathway as the missing regulator of cytokinesis in the C. elegans early embryo.