Ultrafast 2D NMR spectroscopy using a continuous spatial encoding of the spin interactions
- 作者：Shrot ; Yoav ; Shapira ; Boaz ; Frydman ; Lucio
- 关键词：Ultrafast 2D NMR ; Spatial encoding ; Chirped RF irradiation ; Protein HSQC NMR
- 刊名：Journal of Magnetic Resonance
- 出版年：2004
- 期刊代码：126_10907807
- 类别：ch
- 出版时间：November, 2004
- 卷：171
- 期：1
- 页码：163-170
- 文件大小：607 K
摘要
A new protocol for acquiring multidimensional NMR spectra within a single scan is introduced and illustrated. The approach relies on applying a pair of frequency-chirped excitation and storage pulses in combination with echoing magnetic field gradients, in order to impart the kind of linear spatial encoding of the NMR interactions that is required by ultrafast 2D NMR spectroscopy. It is found that when dealing with 2D NMR experiments involving a t1 amplitude-modulation of the spin evolution, such continuous encoding scheme presents a number of advantages over alternatives employing discrete excitation pulses. From an experimental standpoint this is mainly reflected by the use of a single pair of bipolar gradients during the course of the indirect-domain encoding, as opposed to the numerous (and more intense) gradient echoes required so far. In terms of the spectral outcome, main advantages of the continuous spatial encoding scheme are the avoidance of 芒芒芒ghost peaks芒芒芒 and of 芒芒芒enveloping effects芒芒芒 associated to the discrete excitation mode. The principles underlying this new spatial encoding protocol are derived, and its applicability is demonstrated with homo- and heteronuclear 2D ultrafast NMR applications on small molecule and on protein samples.