The spider acylpolyamine Mygalin is a potent modulator of innate immune responses

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• 作者：Diego Gabriel Mafra^a ; Pedro Ismael da Silva Jr.^b ; Cynthia Soares Galhardo^a ; Rafael Nassar^a ; Sirlei Daffre^c ; Maria N. Sato^d ; Monamaris M. Borges^a ; ^{monaborges@butantan.gov.br}
• 关键词：Acylpolyamine ; Mygalin ; Lymphocyte ; Macrophage ; Nitric oxide ; Cytokine ; Innate immunity
• 刊名：Cellular Immunology
• 出版年：2012
• 期刊代码：279_00088749
• 类别：med
• 出版时间：January-February, 2012
• 卷：275
• 期：1-2
• 页码：5-11
• 文件大小：538 K

摘要

Mygalin is an antibacterial molecule isolated from the hemocytes of the spider Acanthoscurria gomesiana. It was identified as bis-acylpolyamine spermidine. We evaluated the modulator effects of synthetic Mygalin in the innate immune response. We demonstrate that Mygalin induces IFN-纬 synthesis by splenocytes increasing the nitrite secretion by splenocytes and macrophages. A specific inhibitor of iNOS abrogated Mygalin-induced nitrite production in macrophages independent of IFN-纬 activation. In addition, Mygalin-activated macrophages produced TNF-伪 but not IL-1尾, demonstrating that Mygalin does not act directly on the inflammasome. Furthermore, this compound did not affect spontaneous or Concanavalin A-induced proliferative responses by murine splenocytes and did not induce IL-5 or apoptosis of splenocytes or bone marrow-derived macrophages. These data provide evidence that Mygalin modulates the innate immune response by inducing IFN-纬 and NO synthesis. The combined immune regulatory and antibacterial qualities of Mygalin should be explored as a strategy to enhance immune responses in infection.