In vitro lethal effect of ajowan (Trachyspermum ammi L.) essential oil on hydatid cyst protoscoleces

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• 作者：Mohammad Moazeni^a ; ^{moazeni@shirazu.ac.ir} ; Mohammad Jamal Saharkhiz^b ; Ali Akbar Hosseini^c
• 关键词：Hydatid cyst ; Trachyspermum ammi ; Ajowan ; Essential oil ; Thymol ; 纬-Terpinene ; p-Cymene ; Scolicidal
• 刊名：Veterinary Parasitology
• 出版年：2012
• 期刊代码：107_03044017
• 类别：abs
• 出版时间：8 June, 2012
• 卷：187
• 期：1-2
• 页码：203-208
• 文件大小：442 K

摘要

Various chemical scolicidal agents have been used for inactivation of hydatid cyst protoscolices, but most of them are associated with adverse side effects. Since ajowan (Trachyspermum ammi) has been shown to have a number of medicinal properties, in this study the scolicidal effect of the essential oil (EO) from the fruits of this herbal plant was investigated. Ajowan EO was obtained by hydrodistillation method. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were employed to determine the chemical composition of the EO. Protoscoleces were exposed to various concentrations of EO (3, 5 and 10 mg/mL) for 10, 20, 30, and 60 min. Viability of protoscolices was confirmed by 0.1%eosin staining. A total of 18 compounds representing 99.54%of the total oil, were identified. Thymol (50.07%), 纬-terpinene (23.92%), and p-cymene (22.9%) were found to be the major EO constituents. While the mortality rate of protoscolices in the control group was 6.67%, scolicidal power of ajowan EO at concentration of 3 mg/mL was 31.34, 35.98, 45.17, and 51.58%after 10, 20, 30, and 60 min, respectively. The EO at concentration of 5 mg/mL killed 51.89, 72.20, 88.64, and 100%of protoscolices after 10, 20, 30, and 60 min, respectively. One hundred percent scolicidal activity was observed with ajowan EO at concentration of 10 mg/mL after 10 min of exposure. The results of this study revealed that the EO of ajowan is rich in thymol, 纬-terpinene and p-cymene, has high scolicidal power and it may be used as a natural scolicidal agent.