Engineered endothelial cell adhesion via VCAM1 and E-selectin antibody-presenting alginate hydrogels
- 作者：Marjan Rafat ; Lisa S. Rotenstein ; Jennifer L. Hu ; Debra T. Auguste ; auguste@seas.harvard.edu
- 关键词：Hydrogels ; Calcium alginate ; Cell adhesion molecules ; Cell adhesion ; Cooperativity
- 刊名：Acta Biomaterialia
- 出版年：2012
- 期刊代码：1_17427061
- 类别：ce
- 出版时间：July, 2012
- 卷：8
- 期：7
- 页码：2697-2703
- 文件大小：946 K
摘要
Materials that adhere to the endothelial cell (EC) lining of blood vessels may be useful for treating vascular injury. Cell adhesion molecules (CAMs), such as endothelial leukocyte adhesion molecule-1 (E-selectin) and vascular cell adhesion molecule-1 (VCAM1), modulate EC-leukocyte interactions. In this study, we mimicked cell-cell interactions by seeding cells on alginate hydrogels modified with antibodies against E-selectin and VCAM1, which are upregulated during inflammation. ECs were activated with interleukin-1伪 to increase CAM expression and subsequently seeded onto hydrogels. The strength of cell adhesion onto gels was assessed via a centrifugation assay. Strong, cooperative EC adhesion was observed on hydrogels presenting a 1:1 ratio of anti-VCAM1:anti-E-selectin. Cell adhesion was stronger on dual functionalized gels than on gels modified with anti-VCAM1, anti-E-selectin or the arginine-glycine-aspartic acid (RGD) peptide alone. Anti-VCAM1:anti-E-selectin-modified hydrogels may be engineered to adhere the endothelium cooperatively.