Structural characterization of the carbohydrate backbone of the lipooligosaccharide of the marine bacterium Arenibacter certesii strain KMM 3941^T

详细信息	查看全文 | 推荐本文 |

• 作者：Alba Silipo ; Antonio Molinaro ; Evgeny L. Nazarenko ; Luisa Sturiale ; Domenico Garozzo ; Raisa P. Gorshkova ; Olga I. Nedashkovskaya ; Rosa Lanzetta and Michelangelo Parrilli
• 关键词：Arenibacter certesii ; Lipopolysaccharide ; Core region ; Marine bacteria ; 2 ; 3-Diamino-2 ; 3-dideoxy-d-glucose
• 刊名：Carbohydrate Research
• 出版年：2005
• 期刊代码：15_00086215
• 类别：ch
• 出版时间：2005
• 卷：340
• 期：16
• 页码：2540-2549
• 文件大小：301 K

摘要

The structure of the carbohydrate backbone of the lipooligosaccharide (LOS) of the marine bacterium Arenibacter certesii strain KMM 3941^T has been elucidated. The structure was obtained by means of compositional analysis, matrix-assisted laser desorption/ionization mass spectrometry and complete ¹H and ¹³C and ³¹P NMR spectroscopy. It shows novel and interesting aspects and is the first description of Arenibacter lipopolysaccharides. Strong and mild alkaline treatments, to fully deacylate and only to O-deacylate the LOS were performed in order to determine the core structure. The core consists of a mixture of species differing by the presence of a non-stoichiometric α-d-rhamnose residue. The Kdo unit is substituted at O-5 by α-mannose and at O-4 by a α-galactosyluronic acid phosphate. The lipid A is constituted by a bis-phosphorylated disaccharide unit composed by a 2,3-diamino-2,3-dideoxy-β-d-glucopyranose (DAG) residue as non-reducing end and a GlcN as reducing end.