Production of the monoclonal antibody against Sudan 2 for immunoassay of Sudan dyes in egg

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• 作者：Jing Liu^a ; ¹ ; Huicai Zhang^a ; ¹ ; Dongsheng Zhang^b ; Feng Gao^a ; Jianping Wang^a ; ^{wjpcau@yahoo.com.cn}
• 关键词：Sudan dyes ; Sudan 2 ; Monoclonal antibody ; Enzyme-linked immunosorbent assay ; Egg
• 刊名：Analytical Biochemistry
• 出版年：2012
• 期刊代码：93_00032697
• 类别：imm
• 出版时间：15 April, 2012
• 卷：423
• 期：2
• 页码：246-252
• 文件大小：387 K

摘要

Many methods have been reported to determine the residues of Sudan dyes in food samples. Among the reported methods, enzyme-linked immunosorbent assay (ELISA) was a frequently used practical screen tool. In this study, a novel hapten of Sudan 2 was synthesized by coupling 4-amino-3-methylbenzoic acid to 尾-naphthol, and the monoclonal antibody against Sudan 2 was produced. The obtained antibody can recognize Sudan 1, 2, 3, and 4, Sudan red G, and Para red simultaneously. After evaluation of different coating antigens, a heterologous indirect competitive ELISA was then developed to determine the six Sudan dyes in egg. The cross-reactivities for the six analytes were in a range of 63%to 100%, and the limits of detection were in a range of 0.2 to 0.5 ng/g, depending on the compound. Intra- and interassay recoveries from the standard fortified blank eggs were in a range of 71.7%to 97.6%with coefficients of variation lower than 17.1%.