Techniques: High-throughput measurement of intracellular Ca²⁺ – back to basics

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• 作者：Monteith ; Gregory R. ; Bird ; Gary St. J.
• 刊名：Trends in Pharmacological Sciences
• 出版年：2005
• 期刊代码：261_01656147
• 类别：med
• 出版时间：April, 2005
• 卷：26
• 期：4
• 页码：218-223
• 文件大小：335 K

摘要

High-throughput screening techniques continue to provide important tools to the pharmaceutical industry for the efficient identification of drug leads. However, high-throughput techniques are now being exploited to address a variety of pharmacological and cellular signaling research questions, including the regulation and role of intracellular Ca²⁺ in a plethora of biological systems. Although an awareness of specific assay conditions is crucial for reliable and reproducible measurements of intracellular free Ca²⁺ whichever system of study is used, the complex temporal nature of Ca²⁺ signals has posed some unique limitations for its measurement in high-throughput mode. Progress in high-throughput design has overcome many of these limitations and will complement other technical approaches to understanding the underlying regulation and role of intracellular Ca²⁺.