- 作者：D Jaya Kumar ; shanthijaya02@gmail.com ; R Jaya Santhi
- 关键词：DPPH ; Morinda pubescens ; Antioxidant ; HepG2 ; Caspase ; MTT assay
- 刊名：Asian Pacific Journal of Tropical Medicine
- 出版年：2012
- 期刊代码：pca68_19957645
- 类别：med
- 出版时间：May, 2012
- 卷：5
- 期：5
- 页码：362-366
- 文件大小：351 K
Objective
To evaluate the antioxidant and cytotoxic effects of hexane extract of Morinda pubescens leaves and to find the primary bioactive compound responsible for antioxidant and cytotoxic activities.Methods
The individual compounds were isolated using column chromatography and were characterized by spectroscopic techniques. The antioxidant activity was evaluated for all individual isolated compounds by DPPH method using L-Ascorbic acid as standard and cytotoxicity was assessed for the extract and the hyoscyamine by MTT assay, caspase test and RT-PCR study.
Results
The antioxidant activity of the isolated compounds and the extract increased as the concentration increased. One of the isolated compound hyoscyamine showed the high antioxidant activity. The extract and the hyoscyamine dose-dependently decreased the cell viability in HepG2 cells. Hyoscyamine induced caspase-3 mediated apoptosis. Up regulation of p53 gene expression provides cue for apoptotic activity of hyoscyamine.
Conclusions
The results indicate that hexane extract possessed potent antioxidant and cytotoxic activity and hyoscyamine is the principal bioactive compound in hexane extract.