Similarity in moth-fly specific larvicidal activity between two serologically unrelated Bacillus thuringiensis strains
- 作者：Higuchi ; Kazuhiko ; Saitoh ; Hiroyuki ; Mizuki ; Eiichi ; Ohba ; Michio
- 关键词：Bacillus thuringiensis ; Parasporal inclusion ; Larvicidal activity ; Moth-fly specificity ; Telmatoscopus albipunctatus
- 刊名：FEMS Microbiology Letters
- 出版年：1998
- 期刊代码：34_03781097
- 类别：imm
- 出版时间：December 15, 1998
- 卷：169
- 期：2
- 页码：213-218
- 文件大小：946 K
摘要
Parasporal inclusions of a Bacillus thuringiensis isolate designated 92-KU-105-9 (H14/19) exhibited unusual larvicidal activity, specific for the moth-fly, Telmatoscopus albipunctatus (Diptera: Psychodidae), similar to that of a previously reported B. thuringiensis serovar leesis (H33) strain. The LC50 value of the purified inclusions was 4.92 μg ml−1 for the moth-fly larvae, while no mortality was shown in the mosquitoes Culex pipiens molestus and Anopheles stephensi, at protein concentrations up to 10 mg ml−1. Morphologically, the inclusion was a homogeneous globular body surrounded by an electron-dense, thick envelope. Multilamellar inner structure was evident between envelope membrane and inclusion matrix. SDS-PAGE revealed that the inclusions consist of five proteins with molecular masses of 72, 70, 68, 56 and 30 kDa. These proteins cross-reacted with the antibodies against inclusion proteins of the serovar leesis strain. High homologies existed in N-terminal amino acid sequences between the three major proteins (72, 70 and 68 kDa) and the two established protein classes, Cry4A and Cry10A.