Effect of IL-10 antisense gene therapy in severely burned mice intradermally infected with MRSA

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• 作者：Akira Asai^a ; Mari Kogiso^a ; Makiko Kobayashi^a ; ^b ; David N. Herndon^b ; Fujio Suzuki^a ; ^b ; ^{fsuzuki@utmb.edu}
• 关键词：Immunosuppression ; Macrophages ; MRSA intradermal infection
• 刊名：Immunobiology
• 出版年：2012
• 期刊代码：3_01712985
• 类别：imm
• 出版时间：July, 2012
• 卷：217
• 期：7
• 页码：711-718
• 文件大小：569 K

摘要

The effect of IL-10 antisense oligodeoxynucleotides (ODN) on the susceptibility of burned mice to intradermal (i.d.) infection of methicillin-resistant Staphylococcus aureus (MRSA) was studied. Abscesses formed and sepsis did not develop in normal mice infected i.d. with 10⁸ CFU/mouse of MRSA. Similarly, sepsis caused by MRSA i.d. infection did not develop and abscesses formed in burned mice treated with IL-10 antisense ODN. However, all of the burned mice treated with scrambled ODN (control group) died by infectious complications stemming from MRSA i.d. infection, and an MRSA-abscess did not form in these mice. Macrophages (M蠒) isolated from the infection site tissue of burned mice that were treated with IL-10 antisense ODN were identified as M1M蠒, while M蠒 isolated from burned mice that were treated with scrambled ODN were shown to be M2M蠒. MRSA-abscesses formed in burned mice inoculated with M1M蠒, and these mice resisted a lethal dose of MRSA i.d. infection. However, an abscess did not form, and sepsis caused by MRSA i.d. infection developed in burned mice that were inoculated with M2M蠒. These results indicate that severely burned mice treated with IL-10 antisense ODN are resistant against i.d. infection with MRSA. M1M蠒 appeared in the infection site tissues of severely burned mice that were treated with IL-10 antisense ODN may play a role on the abscess formation and inhibiting sepsis caused by MRSA i.d. infection.