Fungal bioconversion of 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4-dichlorophenol (2,4-DCP)
- 作者：T. Vroumsia ; R. Steiman ; F. Seigle-Murandi ; J.-L. Benoit-Guyod and Groupe pour l’ ; É ; tude du Devenir des Xé ; nobiotiques dans l’ ; Environnement (GEDEXE)
- 关键词：2 ; 4-Dichlorophenoxyacetic acid (2 ; 4-D) ; 2 ; 4-Dichlorophenol (2 ; 4-DCP) ; Bioconversion ; Biodegradation ; Fungi ; Micromycetes
- 刊名：Chemosphere
- 出版年：2005
- 期刊代码：15_00456535
- 类别：env
- 出版时间：2005
- 卷：60
- 期：10
- 页码：1471-1480
- 文件大小：165 K
摘要
Ninety strains of fungi from the collection of our mycology laboratory were tested in Galzy and Slonimski (GS) synthetic liquid medium for their ability to degrade the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) and its by-product, 2,4-dichlorophenol (2,4-DCP) at 100 mg l−1, each. Evolution of the amounts of each chemical in the culture media was monitored by HPLC. After 5 days of cultivation, the best results were obtained with Aspergillus penicilloides and Mortierella isabellina for 2,4-D and with Chrysosporium pannorum and Mucor genevensis for 2,4-DCP. The data collected seemed to prove, on one hand, that the strains responses varied with the taxonomic groups and the chemicals tested, and, on the other hand, that 2,4-D was less accessible to fungal degradation than 2,4-DCP. In each case, kinetics studies with the two most efficient strains revealed that there was a lag phase of 1 day before the onset of 2,4-D degradation, whereas there was none during 2,4-DCP degradation. Moreover, 2,4-DCP was detected transiently during 2,4-D degradation. Finally, M. isabellina improved its degradation potential in Tartaric Acid (TA) medium relative to GS and Malt Extract (ME) media.