Simultaneous production of anthocyanin and triterpenoids in suspension cultures of Perilla frutescens

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• 作者：Wang ; J.W. ; Xia ; Z.H. ; Chu ; J.H. ; Tan ; R.X.
• 关键词：Cell suspension culture ; Elicitation ; Perilla frutescens ; Labiatae ; Anthocyanin ; Triterpenoids
• 刊名：Enzyme and Microbial Technology
• 出版年：2004
• 期刊代码：26_01410229
• 类别：ce
• 出版时间：June 8, 2004
• 卷：34
• 期：7
• 页码：651-656
• 文件大小：102 K

摘要

When cultivated in Murashige & Skoog medium supplemented with 0.2mgl⁻¹ 2,4-dichlorophenoxy acetic acid and 0.5mgl⁻¹ 6-benzyladenine, Perilla frutescens cells in suspension culture grew rapidly reaching about 13.6g dry wtl⁻¹ after 12 days. The cell line produced both anthocyanin 0.9gl⁻¹ and triterpenoids: 16mgl⁻¹ oleanolic acid (OA), 25mgl⁻¹ ursolic acid (UA) and 14mgl⁻¹ tormentic acid (TA). When P. frutescens cells of 7-day-old cultures were exposed to a yeast elicitor at 0.5–5%(v/v) for 7 days, it was found that anthocyanin content peaked at 10.2%of dry weight with yeast elicitor at 1%(v/v) whereas the maximum production of oleanolic acid and ursolic acid in cultures treated with 2%(v/v) yeast elicitor was 19 and 27mgl⁻¹, a 46 and 24%increase over the control, respectively. This is the first report of simultaneous production of both anthocyanin and triterpenoids in a single culture system.