The effect of nitrate and ammonium concentrations on growth and alkaloid accumulation of Atropa belladonna hairy roots
- 作者：Bensaddek ; Lamine ; Gillet ; Franç ; oise ; Saucedo ; José ; Edmundo Nava ; Fliniaux ; Marc-André
- 关键词：Atropa belladonna ; Ammonium ; Hairy roots ; Hyoscyamine ; Nitrate ; Scopolamine ; C8 ; Atropa belladonna hairy root clone transformed with the genes rolA ; rolB and ; rolC ; C4 ; Atropa belladonna hairy root clone transformed with Agrobacterium rhizogenes 15834 ; d.
- 刊名：Journal of Biotechnology
- 出版年：2001
- 期刊代码：53_01681656
- 类别：imm
- 出版时间：January 23, 2001
- 卷：85
- 期：1
- 页码：35-40
- 文件大小：77.3 K
摘要
The growth, the alkaloid production, as well as the scopolamine/hyoscyamine ratio of two clones of belladonna hairy roots were studied. The effects of nitrate and ammonium concentrations on these cultures were investigated. A rise in ammonium concentration caused the decline of the hairy roots, while nitrate had a marked effect on the alkaloid content. The alkaloid production obtained with 15.8 mM of NO3− and 20.5 mM of NH4+ was 1.2–1.4 times higher than that obtained when the roots were grown in the standard Murashige and Skoog medium (MS medium, 39.5 mM of NO3− and 20.5 mM of NH4+). The nitrate and ammonium concentrations in the culture medium also had a strong influence on the scopolamine/hyoscyamine ratio. When nitrate or ammonium concentrations were raised, that ratio also was increased 2–3-fold. The hairy root clones originating from transformations with two distinct strains of Agrobacterium had similar responses.