Hypoxia-induced secretion of macrophage migration-inhibitory factor from MCF-7 breast cancer cells is regulated in a hypoxia-inducible factor-independent manner
- 作者：Mona Larsen ; Simon Tazzyman ; Eva L. Lund ; Nanna Junker ; Claire E. Lewis ; Paul E.G. Kristjansen ; Craig Murdoch
- 关键词：Macrophage migration-inhibitory factor ; Hypoxia ; Hypoxia-inducible factor ; Breast cancer
- 刊名：Cancer Letters
- 出版年：2008
- 期刊代码：44_03043835
- 类别：med
- 出版时间：8 July 2008
- 卷：265
- 期：2
- 页码：239-249
- 文件大小：388 K
摘要
The cytokine MIF is over-expressed in tumors and is associated with tumor proliferation, angiogenesis and metastasis. Hypoxia, a hallmark feature of tumors, increases MIF expression from tumor cells. We examined the role of hypoxia-inducible transcription factors on MIF secretion from MCF-7 breast carcinoma cells. Secretion of MIF was induced by hypoxia after 24 h but up-regulation of MIF mRNA was minimal. Inhibition of HIF-1
, HIF-2, NF-κB and C/EBPβ using siRNA had no effect on hypoxia-induced MIF secretion. However, inhibition of transcription and translation significantly decreased MIF production, suggesting that hypoxia-induced secretion of MIF in MCF-7 cells is via an alternative pathway.
, HIF-2, NF-κB and C/EBPβ using siRNA had no effect on hypoxia-induced MIF secretion. However, inhibition of transcription and translation significantly decreased MIF production, suggesting that hypoxia-induced secretion of MIF in MCF-7 cells is via an alternative pathway.