DNA microarray for genotyping multidrug-resistant Pseudomonas aeruginosa clinical isolates
- 作者：Jan Weile ; Rolf D. Schmid ; Till T. Bachmann ; Milorad Susa ; Cornelius Knabbe
- 关键词：Pseudomonas aeruginosa ; DNA microarray ; Antibiotic resistance ; Nosocomial infections ; Multidrug resistance
- 刊名：Diagnostic Microbiology and Infectious Disease
- 出版年：2007
- 期刊代码：79_07328893
- 类别：med
- 出版时间：November 2007
- 卷：59
- 期：3
- 页码：325-338
- 文件大小：1042 K
摘要
The management of infections with multidrug-resistant Pseudomonas aeruginosa needs fast and reliable methods of antibiotic susceptibility testing for a therapy improvement. For this purpose, we developed a DNA microarray for genotyping antibiotic resistance and a few virulence factors. The array covers mutations in the efflux regulators mexR, nfxB, mexT, gyrase gyrA, and parC, as well as plasmid-encoded vim, imp, oxa, aph, aac, and aad genes, and virulence-associated mucA and exoU, exoT, and exoS genes, respectively. The whole procedure can be performed in less than 5 h and consists of DNA isolation, target gene amplification, fluorescence labeling, fragmentation, and array hybridization. Concerning the genotype–phenotype comparison in the test collection, the coverage of relevant resistance determinants for antibiotics used in a calculated therapy of critical ill patients was 87.8%.