The transcription factor c-Myb affects pre-mRNA splicing
- 作者：Christophe Orvain ; Vilborg Matre ; Odd S. Gabrielsen
- 关键词：Alternative splicing ; Transcription factor ; c-Myb ; v-Myb ; E1A pre-mRNA
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2008
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：25 July 2008
- 卷：372
- 期：2
- 页码：309-313
- 文件大小：456 K
摘要
c-Myb is a transcription factor which plays a key role in haematopoietic proliferation and lineage commitment. We raised the question of whether c-Myb may have abilities beyond the extensively studied transcriptional activation function. In this report we show that c-Myb influences alternative pre-mRNA splicing. This was seen by its marked effect on the 5′-splice site selection during E1A alternative splicing, while no effect of c-Myb was observed when reporters for the 3′-splice site selection or for the constitutive splicing process were tested. Moreover, co-immunoprecipitation experiments provided evidence for interactions between c-Myb and distinct components of the splicing apparatus, such as the general splicing factor U2AF65 and hnRNPA1 involved in the 5′-splice site selection. The effect on 5′-splice site selection was abolished in the oncogenic variant v-Myb. Altogether, these data provide evidence that c-Myb may serve a previously unappreciated role in the coupling between transcription and splicing.