Anti-diabetic effects of Korean red pepper via AMPK and PPAR-纬 activation in C2C12 myotubes
- 作者：Hye Jeong Yang ; Dai-Ja Jang ; Jin-Taek Hwang ; jthwang@kfri.re.kr
- 关键词：Korean red pepper ; Anti-diabetes ; AMP-activated protein kinase ; Peroxisome proliferator-activated receptor gamma ; C2C12 myotube
- 刊名：Journal of Functional Foods
- 出版年：2012
- 期刊代码：P18_17564646
- 类别：bio
- 出版时间：April, 2012
- 卷：4
- 期：2
- 页码：552-558
- 文件大小：564 K
摘要
In the present study, the anti-diabetic properties and mechanisms underlying the actions of Korean red pepper using C2C12 myotube were evaluated. An ethanol (70%) extract of Korean red pepper (Ekrp) markedly increased 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose uptake in a concentration-dependent manner. To examine the mechanism by which Ekrp increased glucose uptake in C2C12 myotube, the phosphorylation levels of AMP-activated protein kinase (AMPK) and Acetyl-CoA carboxylase (ACC), a marker of AMPK activity were investigated using western blot analysis. Ekrp significantly increased AMPK and ACC phosphorylations in a dose dependent manner. Moreover, we also examined whether the transcriptional activity of peroxisome proliferator-activated receptor gamma (PPAR-纬) was involved in Ekrp-stimulated glucose uptake. Ekrp increased the transactivation of PPAR-纬 in a concentration-dependent manner. The total phenolic compound content of Ekrp was measured (508.97 卤 24.93 mg/100 g), and several major compounds contents such as capsaicin and dihydrocapsaicin were also measured. Finally, we investigated the anti-obesity properties of Ekrp by using 3T3-L1 adipocyte differentiation systems. Ekrp did not inhibit adipocyte differentiation. These results suggest that Korean red pepper exhibits anti-diabetic effects via activation of PPAR-纬 and AMPK, and that Korean red pepper may be useful for preventing diabetes.