miR-20a promotes migration and invasion by regulating TNKS2 in human cervical cancer cells

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• 作者：Hong-Wei Kang¹ ; Fang Wang¹ ; Qian Wei¹ ; Yi-Fan Zhao ; Min Liu ; Xin Li ; Hua Tang ; ^{htang2002@yahoo.com}
• 关键词：miRNA ; microRNA ; miR-20a ; microRNA-20a ; TNKS2 ; Tankyrase 2 ; UTR ; untranslated region ; ASO ; antisense oligonucleotide ; EGFP ; enhanced green fluorescence protein ; GAPDH ; glyceraldehyde phosphate dehydrogenase ; PARP ; poly (ADP-ribose) polymerase
• 刊名：FEBS Letters
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：23 March, 2012
• 卷：586
• 期：6
• 页码：897-904
• 文件大小：1247 K

摘要

miR-20a is an important member of the miR-17-92 cluster, and its real function in cervical cancer cells is unknown. Our study demonstrated that miR-20a was upregulated in cervical cancer tissues. Overexpression of miR-20a in cervical cancer-derived cell lines, HeLa and C-33A, enhanced long-term cellular proliferation, migration and invasion, whereas inhibition of miR-20a suppressed those functions. We also confirmed that oncogenic TNKS2 is directly upregulated by miR-20a. Furthermore, suppression of TNKS2 expression could inhibit colony formation, migration and invasion of cervical cancer cells. Therefore, we concluded that miR-20a can promote migration and invasion of cervical cancer cells through the upregulation of TNKS2.