Effects of Natriuretic Peptides (ANP, BNP, CNP) on Catecholamine Synthesis and TH mRNA Levels in PC12 Cells
- 作者：Takekoshi ; Kazuhiro ; Ishii ; Kiyoaki ; Isobe ; Kazumasa ; Nomura ; Fumio ; Nammoku ; Toru ; Nakai ; Toshiaki
- 关键词：epithelial Na+ channel ; mRNA expression ; polymerase chain reaction ; pregnancy ; rat pheochromocytoma PC12 cells
- 刊名：Life Sciences
- 出版年：2000
- 期刊代码：62_00243205
- 类别：imm
- 出版时间：April 21, 2000
- 卷：66
- 期：22
- 页码：PL303-PL311
- 文件大小：575 K
摘要
Atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) are present in adrenal chromaffin cells and are co-secreted with catecholamines suggesting that these natriuretic peptides (NPs) may modulate functions of chromaffin cells in an autocrine and/or paracrine manner. Therefore, we investigated the effects of NPs on tyrosine hydroxylase (TH: a rate-limiting enzyme in biosynthesis of catecholamine) mRNA in rat pheochromocytoma PC12 cells. It was also determined whether the cyclic GMP/ cGMP-dependent protein kinase (cGMP/PKG) pathway was involved in theses effects. Finally, we examined the effects of NPs on intracellular catecholamine content to confirm increase of catecholamine synthesis following TH mRNA induction. NPs (0.1 μ M) induced significant increases of the TH mRNA (ANP = BNP > CNP). Also, the effects of NPs on TH mRNA were mimicked by 8-bromo cyclic GMP (1mM) and were blocked by KT5823 (1 μ M) (inhibitor PKG) or LY-83583 (1 μM) (guanylate cyclase inhibitor). Moreover, NPs were shown to induce significant increases of intracellular catecholamine contents (ANP = BNP > CNP). These findings suggest that NPs induced increases of TH mRNA through cGMP/PKG dependent mechanisms, which, in turn, resulted in stimulation of catecholamine synthesis in PC 12 cells.