A new nanoprobe based on FRET between functional quantum dots and gold nanoparticles for fluoride anion and its applications for biological imaging

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• 作者：Mei Xue¹ ; Xu Wang¹ ; Lili Duan ; Wen Gao ; Lifei Ji ; Bo Tang ; ^{tangb@sdnu.edu.cn}
• 关键词：CdTe quantum dots ; Gold nanoparticles ; FRET ; Fluoride anion ; Living cell
• 刊名：Biosensors and Bioelectronics
• 出版年：2012
• 期刊代码：12_09565663
• 类别：ch
• 出版时间：June-July, 2012
• 卷：36
• 期：1
• 页码：168-173
• 文件大小：366 K

摘要

A new nanoprobe was designed for the fluorescence imaging of fluoride anion (F^{鈭?/sup>) in living cells with high sensitivity and selectivity. The design is based on the fluorescence resonance energy transfer (FRET) between CdTe quantum dots (CdTe QDs) and gold nanoparticles (AuNPs) through the formation of cyclic esters between phenylborinic acid and diol. In the presence of F鈭?/sup>, the boronate ester, a 鈥渉ard acid鈥? strongly reacts with F鈭?/sup>, a 鈥渉ard base鈥? Therefore, the boronate ester is converted to trifluoro borate, which causes the breakage of the linkage and disassembles CdTe QDs from AuNPs, resulting in the fluorescence recovery of the quenched CdTe QDs. The interaction mechanism was investigated by 19FNMR on a model that was constructed by a small molecule and F鈭?/sup>. Quantum chemical calculations also testify the reactivity of boronate ester to F鈭?/sup> and the sensing mechanism. Experimental results show that the increase in fluorescence intensity is proportional to the concentration of F鈭?/sup> in the range of 5.0-45 渭M. The detection limit and the relative standard deviation were 50 nM and 2.6%, respectively. Fluorescence imaging of F鈭?/sup> in macrophages cells indicates good cell membrane penetration ability and low cytotoxicity of the nanoprobe, providing a viable alternative to detection of F鈭?/sup> in biological or environmental samples.}