Using a bifunctional polymer for the functionalization of Fe₃O₄ nanoparticles

详细信息	查看全文 | 推荐本文 |

• 作者：Quan Zhang^a ; Liang Luan^a ; Siliang Feng^a ; Husheng Yan^b ; ^{yanhs@nankai.edu.cn} ; Keliang Liu^a ; ^{keliangliu55@126.com}
• 关键词：Bifunctional polymer ; Functionalization of nanoparticles ; Fe3O4 ; Nuclear delivery ; Tat peptide
• 刊名：Reactive and Functional Polymers
• 出版年：2012
• 期刊代码：92_13815148
• 类别：ch
• 出版时间：March, 2012
• 卷：72
• 期：3
• 页码：198-205
• 文件大小：1279 K

摘要

A bifunctional maleimido-tetra(ethylene glycol)-poly(glycerol monoacrylate) (MAL-TEG-PGA) polymer was synthesized and used as a linker to couple functional biomolecules to iron oxide nanoparticles. The cell-penetrating peptide Tat was chosen as a model ligand and successfully conjugated to the surface of Fe₃O₄ nanoparticles using MAL-TEG-PGA. The Tat-conjugated Fe₃O₄ nanoparticles can be prepared simply by applying the linker to the iron oxide nanoparticles and then coupling the Tat peptide to the maleimide terminus or by coating the nanoparticles with a pre-coupled linker. Cell-uptake studies demonstrated that the Tat peptide was an efficient functional biomolecule to translocate iron oxide nanoparticles into the cell nucleus. Tat-conjugated nanoparticles thus prepared may be useful for drug or gene delivery.