Direct electrochemistry of glucose oxidase immobilized on nanostructured gold thin films and its application to bioelectrochemical glucose sensor

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• 作者：Cuicui Qiu^a ; Xia Wang^b ; Xueying Liu^b ; Shifeng Hou^c ; Houyi Ma^a ; ^{hyma@sdu.edu.cn}
• 关键词：Glucose oxidase (GOx) ; Electron transfer ; Bioelectrochemical sensor ; Gold film ; Electrochemical detection
• 刊名：Electrochimica Acta
• 出版年：2012
• 期刊代码：30_00134686
• 类别：ch
• 出版时间：15 April, 2012
• 卷：67
• 期：Complete
• 页码：140-146
• 文件大小：962 K

摘要

Glucose oxidase (GOx) was stably immobilized via a simple physical adsorption method onto the nanostructured Au thin films fabricated by using electrodeposition and galvanic replacement technology, which provides a facile method to prepare morphology-controllable Au films and also facilitates the preparation and application of enzyme modified electrodes. An obvious advantage of the as-prepared enzyme electrode (denoted as GOx/Au/GCE) is that the nano-Au films provide a favorable microenvironment for GOx and facilitate the electron transfer between the active center of GOx and electrodes. Cyclic voltammetry (CV) results indicate that the immobilized GOx displayed a direct, reversible and surface-confined redox reaction in the phosphate buffer solution. Furthermore, the enzyme modified electrode was used as a glucose bioelectrochemical sensor, exhibiting a linear relationship in the concentration ranges of 2.5-32.5 渭mol L^鈭? and 60-130 渭mol L^鈭? with a detection limit of 0.32 渭mol L^鈭? (S/N = 3) at an applied potential of 鈭?.55 V. Due to the excellent stability, sensitivity and anti-interference ability, the Au thin films are hopeful in the construction of glucose biosensors.