Effects of the organophosphate insecticides phosmet and chlorpyrifos on trophoblast JEG-3 cell death, proliferation and inflammatory molecule production
- 作者：Natalia Guiñ ; azú ; a ; natanien@hotmail.com ; Viviana Renac ; Susana Genti-Raimondic ; Virginia Riveroc ; Gladis Magnarellia ; b
- 关键词：Cp ; chlorpyrifos ; CPM ; counts per minute ; CT ; cytotrophoblasts ; DMEM ; Dulbecco&rsquo ; s modified Eagle&rsquo ; s medium ; HNF1伪 ; hepatic nuclear factor 1 alpha ; IL ; interleukin ; LDH ; lactate dehydrogenase ; mAb ; monoclonal-antibody ; NO ; nitric oxide ; OP ; orga
- 刊名：Toxicology in Vitro
- 出版年：2012
- 期刊代码：48_08872333
- 类别：pha
- 出版时间：April, 2012
- 卷：26
- 期：3
- 页码：406-413
- 文件大小：553 K
摘要
Epidemiological data have associated environmental organophosphate insecticide (OP) exposure during pregnancy with fetal growth deficits. To better understand OP injury that may adversely affect pregnancy, we used the JEG-3 choriocarcinoma cell line, which provide a recognized in vitro model to study placental function. The effects of the OP phosmet (Pm) and chlorpyrifos (Cp) on JEG-3 cells viability, proliferation, cell cycle and inflammatory molecule production were evaluated. Both insecticides affected cellular viability in a concentration- and time-dependent manner, inducing apoptosis and decreasing [3H]-thymidine incorporation. However, only Pm reduced DNA synthesis independently of cellular death and decreased the cell percentage at the S-phase. Unlike apoptosis, TNF伪 production varied with the concentration tested, suggesting that other TNF伪 independent mechanisms might trigger cell death. No induction of the inflammatory molecule nitric oxide was detected. The mRNA levels of pro-inflammatory IL-6, IL-17 and the anti-inflammatory IL-13 cytokines were differentially modulated. These findings show that Pm and Cp generate a specific toxicity signature, altering cell viability and inducing an inflammatory cytokine profile, suggesting that trophoblasts may represent a possible target for OP adverse effects.