Optimization of extracellular glucan production from Pleurotus eryngii and its impact on angiogenesis
- 作者：Ramalingam Shenbhagaraman ; shenbhagaraman@gmail.com ; Loganathan K. Jagadish ; Kishorekumar Premalatha ; Venkatesan Kaviyarasan
- 关键词：Extracellular polysaccharide ; King oyster ; Anti-angiogenesis ; Box&ndash ; Behnken ; CAM assay
- 刊名：International Journal of Biological Macromolecules
- 出版年：2012
- 期刊代码：62_01418130
- 类别：abs
- 出版时间：1 May, 2012
- 卷：50
- 期：4
- 页码：957-964
- 文件大小：1443 K
摘要
Pleurotus eryngii, an edible mushroom with therapeutic potential was optimized using response surface methodology of four-factor Box-Behnken design for maximum mycelial biomass and extracellular glucan (EPS) production. The model predicts to gain a maximal mycelial biomass and extracellular polysaccharide at 39.4 g/l; 36.04 g/l of glucose, 8.27 g/l; 7.51 g/l of yeast extract, pH 6.99; 7.07 and temperature 26.2 掳C; 25.84 掳C, respectively. The validation experiments showed that the model was significant and in close agreement with the model prediction. The evaluation of extracellular polysaccharide on angiogenesis by ex vivo CAM assay showed that there was significant inhibition in neo-vascularization.